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mouse fc receptor blocking agent  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec mouse fc receptor blocking agent
    Mouse Fc Receptor Blocking Agent, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1276 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+fc+receptor+blocking+agent/FcR+Blocking+Reagent%2C+mouse/pmc12757062-199-12-17
    Average 97 stars, based on 1276 article reviews
    mouse fc receptor blocking agent - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Staining:

    Article Title: Apolipoprotein E drives microglia activation in the development of autoimmune uveitis through up-regulation of peptidyl prolyl isomerase F
    Article Snippet: .. For surface receptor staining, the prepared cell suspension was treated with a mouse Fc receptor blocking agent (Miltenyi Biotec, 130-092-575, diluted 1:100) and incubated at 4°C for 20 min, followed by staining with specific antibodies at 4°C in the dark for 20 min. .. Cells were then fixed and permeabilized using a flow cytometry fixation/permeabilization buffer (Thermo Fisher Scientific, 00-5223-56 and 00-5123-43) at room temperature in the dark for 10 min. For intracellular cytokine staining, the cells were subsequently incubated with a flow cytometry permeabilization buffer (Thermo Fisher Scientific, 00-8333-56), followed by the addition of specific antibodies and incubation at room temperature in the dark for 20 min. Acquisition and compensation were performed by Cytek Aurora Spectral Flow Cytometry.

    Suspension:

    Article Title: Apolipoprotein E drives microglia activation in the development of autoimmune uveitis through up-regulation of peptidyl prolyl isomerase F
    Article Snippet: .. For surface receptor staining, the prepared cell suspension was treated with a mouse Fc receptor blocking agent (Miltenyi Biotec, 130-092-575, diluted 1:100) and incubated at 4°C for 20 min, followed by staining with specific antibodies at 4°C in the dark for 20 min. .. Cells were then fixed and permeabilized using a flow cytometry fixation/permeabilization buffer (Thermo Fisher Scientific, 00-5223-56 and 00-5123-43) at room temperature in the dark for 10 min. For intracellular cytokine staining, the cells were subsequently incubated with a flow cytometry permeabilization buffer (Thermo Fisher Scientific, 00-8333-56), followed by the addition of specific antibodies and incubation at room temperature in the dark for 20 min. Acquisition and compensation were performed by Cytek Aurora Spectral Flow Cytometry.

    Blocking Assay:

    Article Title: Apolipoprotein E drives microglia activation in the development of autoimmune uveitis through up-regulation of peptidyl prolyl isomerase F
    Article Snippet: .. For surface receptor staining, the prepared cell suspension was treated with a mouse Fc receptor blocking agent (Miltenyi Biotec, 130-092-575, diluted 1:100) and incubated at 4°C for 20 min, followed by staining with specific antibodies at 4°C in the dark for 20 min. .. Cells were then fixed and permeabilized using a flow cytometry fixation/permeabilization buffer (Thermo Fisher Scientific, 00-5223-56 and 00-5123-43) at room temperature in the dark for 10 min. For intracellular cytokine staining, the cells were subsequently incubated with a flow cytometry permeabilization buffer (Thermo Fisher Scientific, 00-8333-56), followed by the addition of specific antibodies and incubation at room temperature in the dark for 20 min. Acquisition and compensation were performed by Cytek Aurora Spectral Flow Cytometry.

    Incubation:

    Article Title: Apolipoprotein E drives microglia activation in the development of autoimmune uveitis through up-regulation of peptidyl prolyl isomerase F
    Article Snippet: .. For surface receptor staining, the prepared cell suspension was treated with a mouse Fc receptor blocking agent (Miltenyi Biotec, 130-092-575, diluted 1:100) and incubated at 4°C for 20 min, followed by staining with specific antibodies at 4°C in the dark for 20 min. .. Cells were then fixed and permeabilized using a flow cytometry fixation/permeabilization buffer (Thermo Fisher Scientific, 00-5223-56 and 00-5123-43) at room temperature in the dark for 10 min. For intracellular cytokine staining, the cells were subsequently incubated with a flow cytometry permeabilization buffer (Thermo Fisher Scientific, 00-8333-56), followed by the addition of specific antibodies and incubation at room temperature in the dark for 20 min. Acquisition and compensation were performed by Cytek Aurora Spectral Flow Cytometry.



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